Biokonjugasi AmineQDs untuk Penargetan Spesifik Sel Kanker
Abstract
This study aims to develop an AmineQDs bioconjugation technique to enhance specific cancer cell targeting. AmineQDs were synthesized using a solvothermal method, modified with amine groups, and conjugated with folic acid via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide- N-hydroxysuccinimide(EDC-NHS) carbodiimide activation. This bioconjugation process aims to improve the selectivity of QDs toward cancer cells, enabling more effective detection and therapy. Characterization results indicated successful bioconjugation, but the fluorescence of QDs was quenched after conjugation, likely due to electronic structure changes or self-quenching. Further evaluation of reaction conditions, including pH and buffer types, is needed to maintain optical stability. With proper optimization, AmineQDs have the potential to serve as highly accurate and efficient nanotechnology-based cancer diagnostic and therapeutic agents.
Full Text:
PDFReferences
E. Sulistiowati, D. Bisara Lolong, and L. Pangaribuan, “Gambaran Penyebab Kematian Karena Kanker Di 15 Kabupaten/Kota, Indonesia Tahun 2011 (Profiles the Causes of Cancer Deaths in 15 Districs/Municipalities, Indonesia Year 2011),” Buletin Penelitian Sistem Kesehatan, vol. 19, no. 1, pp. 119–125, 2016.
H. Nagai and Y. H. Kim, “Cancer prevention from the perspective of global cancer burden patterns,” Mar. 01, 2017, AME Publishing Company. doi: 10.21037/jtd.2017.02.75.
G. Christyani, M. Carswell, S. Qin, and W. Kim, “An Overview of Advances in Rare Cancer Diagnosis and Treatment,” Jan. 01, 2024, Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/ijms25021201.
E. U. Dewi and N. P. Widari, “Faktor Yang Mempengaruhi Kualitas Hidup Pasien Kanker Pada Masa Pandemi Covid-19 Di Yayasan Kanker Indonesia Surabaya,” 2017.
C. Jin, K. Wang, A. Oppong-Gyebi, and J. Hu, “Application of nanotechnology in cancer diagnosis and therapy - A mini-review,” Int J Med Sci, vol. 17, no. 18, pp. 2964–2973, 2020, doi: 10.7150/ijms.49801.
N. Alrushaid, F. A. Khan, E. A. Al-Suhaimi, and A. Elaissari, “Nanotechnology in Cancer Diagnosis and Treatment,” Pharmaceutics, vol. 15, no. 3, p. 1025, Mar. 2023, doi: 10.3390/pharmaceutics15031025.
A. Sciortino, A. Cannizzo, and F. Messina, “Carbon Nanodots: A Review—From the Current Understanding of the Fundamental Photophysics to the Full Control of the Optical Response,” C (Basel), vol. 4, no. 4, p. 67, Dec. 2018, doi: 10.3390/c4040067.
J. Dai and X. Zhang, “Chemical Regulation of Fluorescence Lifetime,” Chemical & Biomedical Imaging, vol. 1, no. 9, pp. 796–816, Dec. 2023, doi: 10.1021/cbmi.3c00091.
S. Dua, P. Kumar, B. Pani, A. Kaur, M. Khanna, and G. Bhatt, “Stability of carbon quantum dots: a critical review,” RSC Adv, vol. 13, no. 20, pp. 13845–13861, 2023, doi: 10.1039/D2RA07180K.
P. Tandale et al., “Fluorescent quantum dots: An insight on synthesis and potential biological application as drug carrier in cancer,” Biochem Biophys Rep, vol. 26, p. 100962, Jul. 2021, doi: 10.1016/j.bbrep.2021.100962.
A. Hamidu, W. G. Pitt, and G. A. Husseini, “Recent Breakthroughs in Using Quantum Dots for Cancer Imaging and Drug Delivery Purposes,” Nanomaterials, vol. 13, no. 18, p. 2566, Sep. 2023, doi: 10.3390/nano13182566.
Z. Liang, M. B. Khawar, J. Liang, and H. Sun, “Bio-Conjugated Quantum Dots for Cancer Research: Detection and Imaging,” Front Oncol, vol. 11, Oct. 2021, doi: 10.3389/fonc.2021.749970.
S. Smith, K. Goodge, M. Delaney, A. Struzyk, N. Tansey, and M. Frey, “A Comprehensive Review of the Covalent Immobilization of Biomolecules onto Electrospun Nanofibers,” Nanomaterials, vol. 10, no. 11, p. 2142, Oct. 2020, doi: 10.3390/nano10112142.
M. P. F. Santos et al., “Proteases: Importance, Immobilization Protocols, Potential of Activated Carbon as Support, and the Importance of Modifying Supports for Immobilization,” BioTech, vol. 13, no. 2, p. 13, Apr. 2024, doi: 10.3390/biotech13020013.
G. T. Kennedy et al., “Targeted detection of cancer at the cellular level during biopsy by near-infrared confocal laser endomicroscopy,” Nat Commun, vol. 13, no. 1, p. 2711, May 2022, doi: 10.1038/s41467-022-30265-z.
R. H. Tang et al., “A review on advances in methods for modification of paper supports for use in point-of-care testing,” Microchimica Acta, vol. 186, no. 8, p. 521, Aug. 2019, doi: 10.1007/s00604-019-3626-z.
R. Lawrence, M. Watters, C. R. Davies, K. Pantel, and Y.-J. Lu, “Circulating tumour cells for early detection of clinically relevant cancer,” Nat Rev Clin Oncol, vol. 20, no. 7, pp. 487–500, Jul. 2023, doi: 10.1038/s41571-023-00781-y.
S. K. Krishnan, E. Singh, P. Singh, M. Meyyappan, and H. S. Nalwa, “A review on graphene-based nanocomposites for electrochemical and fluorescent biosensors,” RSC Adv, vol. 9, no. 16, pp. 8778–8881, 2019, doi: 10.1039/C8RA09577A.
T. I. Chio and S. L. Bane, “Click Chemistry Conjugations,” 2020, pp. 83–97. doi: 10.1007/978-1-4939-9929-3_6.
K. Naik, S. Chaudhary, L. Ye, and A. S. Parmar, “A Strategic Review on Carbon Quantum Dots for Cancer-Diagnostics and Treatment,” Front Bioeng Biotechnol, vol. 10, May 2022, doi: 10.3389/fbioe.2022.882100.
A. Nair, J. T. Haponiuk, S. Thomas, and S. Gopi, “Natural carbon-based quantum dots and their applications in drug delivery: A review,” Biomedicine & Pharmacotherapy, vol. 132, p. 110834, Dec. 2020, doi: 10.1016/j.biopha.2020.110834.
A. Permadi et al., “Preparation of poly(ethylene glycol) methacrylate coated CuInS 2/ZnS quantum dots and their use in cell staining,” RSC Adv, vol. 2, no. 14, pp. 6018–6022, Jul. 2012, doi: 10.1039/c2ra20187a.
Z. Liang, M. B. Khawar, J. Liang, and H. Sun, “Bio-Conjugated Quantum Dots for Cancer Research: Detection and Imaging,” Front Oncol, vol. 11, Oct. 2021, doi: 10.3389/fonc.2021.749970.
T. Kang et al., “Minimizing the fluorescence quenching caused by uncontrolled aggregation of CdSe/CdS core/shell quantum dots for biosensor applications,” Sens Actuators B Chem, vol. 222, pp. 871–878, Jan. 2016, doi: 10.1016/j.snb.2015.09.036.
Refbacks
- There are currently no refbacks.
